کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10562903 | 969833 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison between the performances of amperometric immunosensors for cholera antitoxin based on three enzyme markers
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
We developed a novel copolymer modified amperometric immunosensor for the detection of cholera antitoxin (anti-CT), by the electropolymerization of pyrrole-biotin and pyrrole-lactitobionamide monomers on platinum or glassy carbon electrodes. In the detection of cholera antitoxin we have used three enzymatic marker detection systems based on HRP-labeled rabbit IgG antibodies, biotinylated polyphenol-oxidase (PPO-B) and biotinylated glucose-oxidase (GOX-B). The comparison of the electro-enzymatic performances of these three configurations with different substrates, clearly shows that the more sensitive amperometric immunosensor was based on HRP with a lower limit of detection of 50Â ng/ml anti-CT using hydroquinone/H2O2 system. The response time for this substrate was in range of 5-30Â s. The HRP-amperometric immunosensor has thus proven to be a very sensitive tool to monitor nanomolar concentrations of anti-CT.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 66, Issue 1, 31 March 2005, Pages 15-20
Journal: Talanta - Volume 66, Issue 1, 31 March 2005, Pages 15-20
نویسندگان
Rodica E. Ionescu, Chantal Gondran, Serge Cosnier, Levi A. Gheber, Robert S. Marks,