کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10565419 971699 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Selective chemical labeling of proteins in living cells
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Selective chemical labeling of proteins in living cells
چکیده انگلیسی
Labeling proteins with fluorophores, affinity labels or other chemically or optically active species is immensely useful for studying protein function in living cells or tissue. The use of genetically encoded green fluorescent protein and its variants has been particularly valuable in this regard. In an effort to increase the diversity of available protein labels, various efforts to append small molecules to selected proteins in vivo have been reported. This review discusses recent advances in selective, in vivo protein labeling based on small molecule ligand-receptor interactions, intein-mediated processes, and enzyme-catalyzed protein modifications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Opinion in Chemical Biology - Volume 9, Issue 1, February 2005, Pages 56-61
نویسندگان
, ,