Synthesis and in vitro cytotoxic activity of novel pyrazolo[3,4-d]pyrimidines and related pyrazole hydrazones toward breast adenocarcinoma MCF-7 cell line

The present work reports the synthesis of novel pyrazolo[3,4-d]pyrimidines and related pyrazole hydrazones and the evaluation of their cytotoxic activity against human breast cancer cell MCF-7. In addition, the effect of selected compounds on the activities of free-radical-metabolizing enzymes as well as the levels of oxidative stress parameters in MCF-7 cells was estimated.