کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10586402 | 981390 | 2011 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Design and synthesis of biotinylated inositol 1,3,4,5-tetrakisphosphate targeting Grp1 pleckstrin homology domain
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کلمات کلیدی
PLCIns(1,3,4,5)P4d-myo-inositol 1,4,5-trisphosphate - D-myo-inositol 1،4،5-trisphosphateIns(1,4,5)P3 - Ins (1،4،5) P3Biotin - بیوتین، ویتامین B7،فاکتور W، کوآنزیم R، ویتامین HSPR - تشدید پلاسمون سطحیSurface plasmon resonance - تشدید پلاسمون سطحیphospholipase C - فسفولیپاز CPleckstrin Homology - همخوانی Pleckstrin
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A bifunctional molecule containing biotin and d-myo-inositol 1,3,4,5-tetrakisphosphate was synthesized. This molecule was designed on the basis of X-ray structure of the complex of d-myo-inositol 1,3,4,5-tetrakisphosphates, Ins(1,3,4,5)P4, and Grp1 PH (general receptor of phosphoinositides pleckstrin homology) domain for the application to the widely employed biotin-avidin techniques. The building block of inositol moiety was synthesized starting with myo-inositol and assembled with the biotin-linker moiety through a phosphate linkage. The equilibrium dissociation constant KD of biotinylated Ins(1,3,4,5)P4 binding of original Grp1 PH domain was 0.14 μM in pull-down analysis, which was comparable to that of unmodified Ins(1,3,4,5)P4. Furthermore, biotinylated Ins(1,3,4,5)P4 had an ability to distinguish Grp1 PH domain from PLCδ1 PH domain. Thus, biotinylated Ins(1,3,4,5)P4 retained the binding affinity and selectivity of original Grp1 PH domain, and realized the intracellular Ins(1,3,4,5)P4 despite a tethering at the 1-phosphate group of inositol.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 19, Issue 22, 15 November 2011, Pages 6833-6841
Journal: Bioorganic & Medicinal Chemistry - Volume 19, Issue 22, 15 November 2011, Pages 6833-6841
نویسندگان
Kensaku Anraku, Teruhiko Inoue, Kenji Sugimoto, Kota Kudo, Yoshinari Okamoto, Takashi Morii, Yasuo Mori, Masami Otsuka,