کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10595254 981861 2012 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Design and synthesis of novel photoaffinity probes for study of the target proteins of oleanolic acid
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Design and synthesis of novel photoaffinity probes for study of the target proteins of oleanolic acid
چکیده انگلیسی
To explore the molecular mechanisms of oleanolic acid, two novel photoaffinity probes were synthesized based on the structure-activity relationship reported previously. Their potency were evaluated in an enzyme inhibition assay against rabbit muscle glycogen phosphorylase a (RMGPa), a known target protein of oleanolic acid. The inhibitory activity of probe 2 was only about two-fold less potent than the mother compound oleanolic acid. The photoaffinity labeling experiments were also performed and two proteins were specifically tagged by probe 2. The results suggest that the synthesized probes could be used as powerful tools to isolate and identify the target proteins of oleanolic acid.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 22, Issue 2, 15 January 2012, Pages 1036-1039
نویسندگان
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