First experimental identification of Ras-inhibitor binding interface using a water-soluble Ras ligand

By combining in the same molecule Ras-interacting aromatic moieties and a sugar, we prepared a water-soluble Ras ligand that binds Ras and inhibits guanine nucleotide exchange. With this compound it was possible to determine experimentally by a 15N-edited HSQC NMR experiment the ligand-Ras binding interface.