Self-assembled nanoparticles based on amphiphilic chitosan derivative and hyaluronic acid for gene delivery

► OD NPs were successfully prepared by complex coacervation process. ► The optimum N/P ratio was found to be 5 with the ideal OCMCS/HA weight ratio of 4. ► The MTT survival assay indicated no significant cytotoxicity. ► Involving HA in NPs was beneficial for the DNA release as well as cellular uptake. ► The transfection efficiency of OHD NPs was 5-fold higher than OD NPs.