کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10601662 | 982350 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The effect of low-frequency ultrasound on the activity and efficiency of a commercial cellulase enzyme
ترجمه فارسی عنوان
اثر اولتراسوند فرکانس پایین بر فعالیت و کارایی آنزیم تجاری سلولاز
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کلمات کلیدی
سلولز پنبه، هیدرولیز، آنزیم سلولاز، سونوگرافی، فعالیت آنزیم، کاهش آزاد سازی قند،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
چکیده انگلیسی
A commercial acidic cellulase enzyme complex was chosen in order to gain detailed information about the effect of low-frequency ultrasound (horn at 40Â kHz) on the enzyme activity. The performance of the enzyme under sonication was also evaluated in a cellulose-cellulase model reaction. The filter paper activity of the enzyme and the yield of the enzyme catalysed hydrolysis were determined as a function of the parameters of the sonicated environment (treatment time, amplitude, with and without a reflector) and compared with the data measured in a non-sonicated bath. Depending on the parameters of the sonication, the enzyme is susceptible to ultrasound and its activity can significantly decrease. Despite the serious reduction of the enzyme activity, the outcome of the enzyme catalysed hydrolysis was always positive, implying that the advantageous effects of sonication impressed on the heterogeneous enzyme reaction always overcome the undesirable enzyme modifying effect of ultrasound.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 98, Issue 2, 6 November 2013, Pages 1483-1489
Journal: Carbohydrate Polymers - Volume 98, Issue 2, 6 November 2013, Pages 1483-1489
نویسندگان
Orsolya Erzsébet Szabó, EmÃlia Csiszár,