کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10601670 | 982350 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Structure and anticoagulant activity of fucosylated glycosaminoglycan degraded by deaminative cleavage
ترجمه فارسی عنوان
ساختار و فعالیت ضد انعقادی فلوکوزیله گلیکواسامینو گلیکان ضعیف شده به وسیله تقسیم دمیامیتی
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کلمات کلیدی
heparin cofactor IIHCIIGalNAPTTEC50UFHLMWHGlcUAGAGDeacetylation degree - Degacetylation درجهdFG - DFGGlucuronic acid - اسید گلوکورونیکSea cucumber - خیار دریاییDeacetylation - دفع آلودگیactivated partial thromboplastin time - زمان ترومبوپلاستین جزئی فعال شده استAnticoagulant activity - فعالیت ضد انعقادhalf maximal effective concentration - نیمه حداکثر غلظت موثرunfractionated heparin - هپارین غیر اشباع شدهlow-molecular-weight heparin - هپارین کم وزن مولکولیGalactosamine - گالاکتوزامینGlycosaminoglycan - گلیکوزآمینوگلیکان
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
چکیده انگلیسی
Fucosylated glycosaminoglycans (FGs) are complex glycosaminoglycans that exhibit potent anticoagulant activity. To study the relationship between molecular size and biological activity, oligosaccharides with (2,5)-anhydro-d-talose units at new reducing ends were prepared by hydrazine deacetylation and nitrous acid depolymerization. The product chemical structures were analyzed by one- and two-dimensional NMR methods. Additionally, anticoagulant activities were evaluated by clotting assay and chromogenic substrate cleavage. The results demonstrated that under mild deacetylation and deaminative cleavage conditions, both products were relatively homogeneous and sulfated fucose branch types and sulfate substituents remained stable. These depolymerized FGs with different molecular sizes had potent intrinsic anticoagulant activities, which were similar to those that were obtained by free-radical depolymerization with similar molecular weights. Decreasing molecular weight may weaken activity but not significantly affect factor Xase and heparin cofactor II (HCII)-mediated thrombin inhibition.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 98, Issue 2, 6 November 2013, Pages 1514-1523
Journal: Carbohydrate Polymers - Volume 98, Issue 2, 6 November 2013, Pages 1514-1523
نویسندگان
Longyan Zhao, Sensen Lai, Rong Huang, Mingyi Wu, Na Gao, Li Xu, Hongbo Qin, Wenlie Peng, Jinhua Zhao,