A polysaccharide from the fungi of Huaier exhibits anti-tumor potential and immunomodulatory effects

► Cholangiocarcinoma is an aggressive and lethal cancer. ► A polysaccharide (W-NTRP) was isolated from Trametes robiniophila (Huaier). ► W-NTRP inhibits the growth of three human cholangiocarcinoma cell lines. ► W-NTRP prompts the splenocytes proliferation. ► W-NTRP stimulates macrophages to produce NO through the up-regulation of iNOS activity.