Evaluation of pretreatment methods for enzymatic saccharification of wheat straw for bioethanol production

► Wheat straw, abundant lignocellulosic feed stock selected for producing ethanol. ► Pretreatment with alkali followed by acid and vice versa for enzyme hydrolysis. ► Pretreatment at low temperature is economically feasible. ► Recovery of maximum sugars by enzymatic hydrolysis for high ethanol yield.