Extraction optimization and bioactivity of exopolysaccharides from Agaricus bisporus

► Agaricus bisporus was used for production of EPS. ► RSM was used for EPS extraction from fermentation broth in submerged culture of Agaricus bisporus. ► Agaricus bisporus EPS showed good antioxidant properties in vitro. ► Agaricus bisporus EPS showed good hypoglycemic activity.