Preparation of low-molecular-weight hyaluronic acid by ozone treatment

► Degradation of native hyaluronic acid by ozone treatment. ► Preparation of low-molecular-weight hyaluronic acid. ► The molecular weight of native hyaluronic acid was reduced from 1535 to 87 kDa. ► The rate of reduction of molecular weight was 94.33%. ► Structure of low-molecular-weight hyaluronic acid was not obviously modified.