Structural characterization of a sulfated glucan isolated from the aqueous extract of Hedysarum polybotrys Hand.-Mazz

► We discovered a novel sulfated polysaccharide from Hedysarum polybotrys Hand.-Mazz. ► SHG is characterized by combination of chemical and instrumental analysis, specially, 2D NMR spectroscopy including β-COSY, TOCSY, HSQC, and HMBC experiments. ► We discussed the molecular weight and chain conformation. ► Backbone chains of SHG were shown to be the linear 1,4-α-d-glucan, with a single 1,4,6-α-d-glucosyl side branching unit approximately every eight residues. ► The biological activities of SHG deserve more an in-depth research in the future.