Isolation and characterization of model homogalacturonans of tailored methylesterification patterns

► We prepared model homogalacturonans (HGs) with tailored patterns of methyl-esterification. ► We manipulated the initial DM prior to enzymatic blockwise demethylesterification. ► HGs with few long deesterified blocks of GalA were generated from a very high DM “mother” HG. ► HGs with more numerous but shorter deesterified blocks of GalA were produced from “mother” HGs of lower DM.