کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10603066 | 982436 | 2011 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Antioxidant activity of gallate-chitooligosaccharides in mouse macrophage RAW264.7 cells
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
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چکیده انگلیسی
In this research, a novel derivative of chitooligosaccharides (COS) was synthesized by covalently linking gallic acid and COS (gallate-COS) via carbodiimide to improve cellular antioxidant activity. The direct intracellular radical scavenging effect of gallate-COS by 2â²,7â²-dichlorofluorescin diacetate (DCFH-DA) method, and the inhibition of oxidation of cellular macromolecules, such as DNA, protein and lipid in mouse macrophages (RAW264.7 cells) were determined. Furthermore, with the treatment of gallate-COS, the expression levels of antioxidant enzymes such as superoxide dismutase (SOD) and glutathione (GSH) were significantly increased according to reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis. In addition, gallate-COS decreased reactive oxygen species induced activation of the nuclear transcription factor (NF-κB). Collectively, these results suggest that gallate-COS can be used as a potential natural compound-based antioxidant in the functional food and pharmaceutical industries.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 84, Issue 4, 2 April 2011, Pages 1282-1288
Journal: Carbohydrate Polymers - Volume 84, Issue 4, 2 April 2011, Pages 1282-1288
نویسندگان
Dai-Hung Ngo, Zhong-Ji Qian, Thanh-Sang Vo, BoMi Ryu, Dai-Nghiep Ngo, Se-Kwon Kim,