کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10603480 | 982479 | 2013 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biodegradation of (1â3)-β-polyglucuronate prepared by TEMPO-mediated oxidation
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
2,2,6,6-Tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation was applied to curdlan to prepare water-soluble sodium (1â3)-β-polyglucuronate, and its biodegradation behavior was then investigated. A bacterial strain (EH621) having the ability to degrade (1â3)-β-polyglucuronate was isolated from soil, and identified as Paenibacillus sp. Strain EH621 cultured with (1â3)-β-polyglucuronate decreased the initial total carbon in the supernatant by approximately 60% within 3 days, showing that (1â3)-β-polyglucuronate can be readily degraded and metabolized by microorganisms present in the natural environment. Hydrolytic enzyme activity was detected in the cell-free extract of EH621, which was highly specific to (1â3)-β-polyglucuronate. Analyses of the enzymatic degradation products revealed that (1â3)-β-polyglucuronate was endolytically degraded to its monomeric unit, glucuronate, via some oligomers and dimers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 96, Issue 1, 1 July 2013, Pages 314-319
Journal: Carbohydrate Polymers - Volume 96, Issue 1, 1 July 2013, Pages 314-319
نویسندگان
Erika Watanabe, Naoto Habu, Akira Isogai,