کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10604663 982530 2011 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Optimum extraction of acidic polysaccharides from the stems of Ephedra sinica Stapf by Box-Behnken statistical design and its anti-complement activity
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Optimum extraction of acidic polysaccharides from the stems of Ephedra sinica Stapf by Box-Behnken statistical design and its anti-complement activity
چکیده انگلیسی
The response surface methodology was employed to study the extraction of acidic polysaccharides from the stem of Ephedra sinica Stapf. The quantitative effects of extraction temperature, time, number and ratio of water to raw material on yield of ephedra acidic polysaccharides were investigated with Box-Behnken design. The experimental data were fitted to three second-order polynomial equations using multiple regression analysis and also analyzed using the appropriate statistical methods. By solving the regression equation and analyzing 3-D plots, the optimum condition was at extraction temperature 100 °C, time 3.5 h, numbers 4 and ratio of water to raw material 13.6. Under these conditions, the experimental extraction yield, polysaccharide yield and uronic acid yield were 49.47 mg/g, 33.25 mg/g, and 22.08 mg/g, which were in good agreement with the predicted values. Ephedra acidic polysaccharide exhibited excellent anti-complement activity in vitro, indicating it could be a potential anti-complement therapeutic agent.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 84, Issue 1, 11 February 2011, Pages 282-291
نویسندگان
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