کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10604742 | 982530 | 2011 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Diethylaminoethyl chitosan induces apoptosis in HeLa cells via activation of caspase-3 and p53 expression
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
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چکیده انگلیسی
In our previous study, diethylaminoethyl chitosan (DEAEC) was found to inhibit the viability of HeLa cells, a human uterine cervix cancer cell line. To identify the anti-proliferation activity of DEAEC, its mode of action was studied in further detail in HeLa cells. After incubation for 24 h, DEAEC had significantly inhibited HeLa cell growth with an IC50 of 23.2 μg/mL and showed strong apoptotic activity. After Hoechst 33258 staining, morphological changes and DNA degradation were observed. Cell cycle analysis and an Annexin V assay by flow cytometry revealed that DEAEC induced apoptosis in the HeLa cells by sub-G1 involving early stage apoptosis. RT-PCR and Western blotting analysis results showed that DEAEC induced apoptosis in the HeLa cells via up-regulation of caspase family enzyme (caspase-3, -8 and -9), p53, and Bax protein expressions, and down-regulation of Bcl-2 protein expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 84, Issue 1, 11 February 2011, Pages 571-578
Journal: Carbohydrate Polymers - Volume 84, Issue 1, 11 February 2011, Pages 571-578
نویسندگان
Sang-Hoon Lee, BoMi Ryu, Jae-Young Je, Se-Kwon Kim,