Caenorhabditis elegans proteins captured by immobilized Galβ1-4Fuc disaccharide units: assignment of 3 annexins

► Preparation of an immobilized Galβ1-4Fuc column. ► Caenorhabditis elegans proteins bound to an immobilized Galβ1-4Fuc column were identified. ► Multiple C. elegans galectins have affinities for Galβ1-4Fuc. ► C. elegans annexins, NEX-1, -2, and -3, interact with Galβ1-4Fuc. ► NEX-1 specifically recognizes Galβ1-4Fuc but not Galβ1-3Fuc or Galβ1-4GlcNAc.