کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10613370 | 986883 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Oligonucleotide-protamine-albumin nanoparticles: preparation, physical properties, and intracellular distribution
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
بیومتریال
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چکیده انگلیسی
Oligodesoxynucleotides (ODNs) or the corresponding phosphorothioates (PTOs) spontaneously form spherical nanoparticles (“proticles”) with protamine in aqueous solutions. The proticles can cross cellular membranes and release the ODNs within the cells. Thus, they represent a potential drug delivery system. The major disadvantages of this system are a lack of stability in salt solutions and its inability to also release PTOs. The present study shows, using PTOs and protamine free base, that these shortcomings can be eliminated by the addition of human serum albumin (HSA) as a third component to the starting mixture. The “ternary” proticles thus obtained contain maximally a few percent of the HSA that was originally present. Nevertheless, they differ from the previously studied “binary” proticles: (1) They are stable in salt solutions for at least several hours. (2) They show a high cellular uptake into murine fibroblasts, and they readily release the PTOs after uptake. The ternary proticles therefore represent a considerable improvement over binary proticles for use as drug delivery systems.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Controlled Release - Volume 103, Issue 1, 2 March 2005, Pages 99-111
Journal: Journal of Controlled Release - Volume 103, Issue 1, 2 March 2005, Pages 99-111
نویسندگان
Vitali Vogel, Dirk Lochmann, Jörg Weyermann, Gottfried Mayer, Christos Tziatzios, Jacomina A. van den Broek, Winfried Haase, Daan Wouters, Ulrich S. Schubert, Jörg Kreuter, Andreas Zimmer, Dieter Schubert,