کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10613447 | 986888 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Improving peptide-based assays to differentiate between vaccination and Mycobacterium bovis infection in cattle using nanoparticle carriers for adsorbed antigens
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
بیومتریال
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The development of diagnostic tests to differentiate between vaccinated animals and those infected with Mycobacterium bovis is required so that test and slaughter control strategies can continue alongside vaccination. In this work, the peptide antigen, ESAT-6, p45, derived from the N-terminal sequence of the ESAT-6 protein, was adsorbed onto a range of microparticulate and nanoparticulate substrates to enhance the in vitro immune response of blood lymphocytes previously sensitised to M. bovis. Two types of hydroxyapatite (HA) nanoparticles (both â¼300 nm in linear dimension), carbonate hydroxyapatite nanospheres (CHA, â¼50 nm), two sizes of polystyrene nanospheres (â¼500 and 40 nm), calcium carbonate microparticles (0.3-1.0 μm) and glass microspheres (1.0-3.0 μm) were incubated in a solution of the peptide in PBS. Peptide adsorption increased on the nanoparticle carriers in the order HA (2.5±0.12%w/w), CHA (4.9±0.12) polystyrene (500 nm, 6.8±0.15%, 40 nm, 9.2±0.07) and these systems exhibited fairly low levels of desorption (approximately 10-15% peptide release) over a 24-h incubation period in PBS at 37 °C. HA, CHA and polystyrene carriers with adsorbed peptide were subsequently tested in the BOVIGAM⢠assay to investigate the efficiency of the immune response of blood lymphocytes in terms of interferon-γ (IFN-γ) production. A general elevation of IFN-γ production resulted for particle-bound peptide relative to free peptide at high peptide concentrations (>10 μg/ml). Only HA-adsorbed peptide resulted in consistently higher immune responses at low peptide concentration (<0.1 μg/ml) compared with the free peptide, indicating that peptide antigens adsorbed to hydroxyapatite nanoparticles may be useful, in diagnostic assays, for differentiating between tuberculosis (TB)-infected and vaccinated animals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Controlled Release - Volume 102, Issue 3, 16 February 2005, Pages 551-561
Journal: Journal of Controlled Release - Volume 102, Issue 3, 16 February 2005, Pages 551-561
نویسندگان
I.Y. Saleem, M. Vordermeier, J.E. Barralet, A.G.A. Coombes,