کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10613504 | 986891 | 2005 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fimbriae of enterotoxigenic Escherichia coli function as a mucosal carrier for a coupled heterologous antigen
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
بیومتریال
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Fimbriae of enterotoxigenic Escherichia coli function as a mucosal carrier for a coupled heterologous antigen Fimbriae of enterotoxigenic Escherichia coli function as a mucosal carrier for a coupled heterologous antigen](/preview/png/10613504.png)
چکیده انگلیسی
Receptor-mediated uptake of orally administered antigen can lead to an antigen-specific immune response, whereas oral administration of most other non-replicating soluble antigens results in the induction of oral tolerance. In the present study, it is shown that fimbriae purified from an F4(K88)+ enterotoxigenic Escherichia coli strain can function as a mucosal carrier molecule for the model antigen human serum albumin (HSA). Glutaraldehyde-coupled F4/HSA conjugates were able to bind F4 receptor positive (F4R+) enterocytes, but not to F4Râ enterocytes. Moreover, oral immunization of F4R+ pigs with F4/HSA conjugates induced a HSA-specific immune response, whereas oral immunization with HSA/HSA conjugates did not. This mucosal carrier function of F4 fimbriae was improved following oral co-administration of the F4/HSA conjugates with the mucosal adjuvant cholera toxin (CT) to F4R+ pigs, since both humoral and cellular HSA-specific responses were significantly increased. In comparison with F4R+ pigs, the HSA-specific response was reduced following oral F4/HSA+CT immunization of F4Râ pigs. This indicates that F4 fimbriae as mucosal carrier and CT as adjuvant synergistically improve the induction of a HSA-specific immune response following oral immunization of pigs. These results could open new perspectives in the development of vaccines against enteropathogens.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Controlled Release - Volume 104, Issue 2, 18 May 2005, Pages 243-258
Journal: Journal of Controlled Release - Volume 104, Issue 2, 18 May 2005, Pages 243-258
نویسندگان
F. Verdonck, V. De Hauwere, J. Bouckaert, B.M. Goddeeris, E. Cox,