کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10613515 | 986891 | 2005 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Improvement of gene delivery mediated by mannosylated dendrimer/α-cyclodextrin conjugates
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
بیومتریال
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Improvement of gene delivery mediated by mannosylated dendrimer/α-cyclodextrin conjugates Improvement of gene delivery mediated by mannosylated dendrimer/α-cyclodextrin conjugates](/preview/png/10613515.png)
چکیده انگلیسی
The purpose of this study is to evaluate in vitro and in vivo gene delivery efficiency of polyamidoamine (PAMAM) starburst dendrimer (generation 2, G2) conjugate with α-cyclodextrin (α-CDE conjugate (G2)) bearing mannose (Man-α-CDE conjugates) with the various degrees of substitution of the mannose moiety (DSM) as a novel non-viral vector in a variety of cells. Man-α-CDE conjugates (DSM 3.3 and 4.9) were found to have much higher gene transfer activity than dendrimer, α-CDE conjugate and Man-α-CDE conjugates (DSM 1.1 and 8.3) in various cells, which are independent of the expression of cell surface mannose receptors. Cellular association of pDNA complexes with dendrimer, α-CDE conjugate and Man-α-CDE conjugate (DSM 3.3) and their cytotoxic effects differed only very slightly. Surface plasmon resonance study demonstrated that the specific binding activity of Man-α-CDE conjugates to concanavalin A was not very strong. Much more conjugation of the mannose moiety to α-CDE conjugates provided unfavorable physicochemical properties of pDNA complexes for gene transfer, e.g. the low interaction with pDNA, the low enzymatic stability of pDNA and the lack of pDNA compaction. Man-α-CDE conjugate (DSM 3.3) provided gene transfer activity higher than dendrimer and α-CDE conjugate in kidney 12 h after intravenous injection in mice. These results suggest the potential use of Man-α-CDE conjugate (DSM 3.3) as a non-viral vector.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Controlled Release - Volume 104, Issue 2, 18 May 2005, Pages 397-413
Journal: Journal of Controlled Release - Volume 104, Issue 2, 18 May 2005, Pages 397-413
نویسندگان
Koki Wada, Hidetoshi Arima, Toshihito Tsutsumi, Yuko Chihara, Kenjiro Hattori, Fumitoshi Hirayama, Kaneto Uekama,