کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10613536 | 986893 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Intracellular delivery of liposome-encapsulated prolidase in cultured fibroblasts from prolidase-deficient patients
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
بیومتریال
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Intracellular delivery of liposome-encapsulated prolidase in cultured fibroblasts from prolidase-deficient patients Intracellular delivery of liposome-encapsulated prolidase in cultured fibroblasts from prolidase-deficient patients](/preview/png/10613536.png)
چکیده انگلیسی
Liposomes were prepared, with a 1:1 PC:Col molar ratio with or without DSPE-PEG, by a thin-film hydration. Ex-vivo experiments were performed, incubating prolidase loaded liposomes with cultured fibroblasts from PD patients and from controls, to determine the amount of active enzyme delivered to cells. Evaluation of liposomes toxicity on cultured skin fibroblasts showed that liposomes did not interfer with cellular growth. Results showed that all the active prolidase encapsulated in the liposomes was completely vehiculated inside fibroblasts after 6 days incubation. SEM analysis suggests that prolidase is vehiculated inside the cell through liposome endocytosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Controlled Release - Volume 102, Issue 1, 20 January 2005, Pages 181-190
Journal: Journal of Controlled Release - Volume 102, Issue 1, 20 January 2005, Pages 181-190
نویسندگان
Paola Perugini, Khaolé Hassan, Ida Genta, Tiziana Modena, Franca Pavanetto, Giuseppe Cetta, Chiara Zanone, Paolo Iadarola, Annalia Asti, Bice Conti,