کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10691633 1019602 2014 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Non-contact High-Frequency Ultrasound Microbeam Stimulation for Studying Mechanotransduction in Human Umbilical Vein Endothelial Cells
ترجمه فارسی عنوان
تحرک میکروبی سونوگرافی بدون تماس با فرکانس بالا برای مطالعه انتقال مکانیکی در سلول های اندوتلیالی ورید غضروف انسانی
کلمات کلیدی
میکروب بی سیم اولتراسوند با فرکانس بالا، مکانیک انتقال، سلولهای اندوتلیال ورید انسان، تصویربرداری فلورسانس کلسیم،
موضوعات مرتبط
مهندسی و علوم پایه فیزیک و نجوم آکوستیک و فرا صوت
چکیده انگلیسی
We describe how contactless high-frequency ultrasound microbeam stimulation (HFUMS) is capable of eliciting cytoplasmic calcium (Ca2+) elevation in human umbilical vein endothelial cells. The cellular mechanotransduction process, which includes cell sensing and adaptation to the mechanical micro-environment, has been studied extensively in recent years. A variety of tools for mechanical stimulation have been developed to produce cellular responses. We developed a novel tool, a highly focused ultrasound microbeam, for non-contact cell stimulation at a microscale. This tool, at 200 MHz, was applied to human umbilical vein endothelial cells to investigate its potential to elicit an elevation in cytoplasmic Ca2+ levels. It was found that the response was dose dependent, and moreover, extracellular Ca2+ and cytoplasmic Ca2+ stores were involved in the Ca2+ elevation. These results suggest that high-frequency ultrasound microbeam stimulation is potentially a novel non-contact tool for studying cellular mechanotransduction if the acoustic pressures at such high frequencies can be quantified.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ultrasound in Medicine & Biology - Volume 40, Issue 9, September 2014, Pages 2172-2182
نویسندگان
, , , , , , , , , ,