Application of polyaniline as enzyme based biosensor

Lactate is determined by the photometric detection of NADH formed in the reaction catalysed by LDH. Studies have been carried out with PANI as a matrix for the immobilization of LDH and its feasibility as a biosensor. The results of the photometric and amperometric measurements conducted on such LDH/PANI electrodes show a response to pyruvate concentration upto 0.45 mM, a response time of 90 s and a shelf life of about two weeks.