Elucidation of (−)-epicatechin metabolites after ingestion of chocolate by healthy humans

► A new method to quantify (−)-epicatechin metabolites was developed and validated. ► The method was applied to biological samples after consumption of dark chocolate. ► The most relevant metabolites were 3′-glucuronide, 3′-sulfate, and methyl sulfates. ► The entire metabolite profile of (−)-epicatechin is described.