کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738085 | 1046682 | 2011 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
GSTM1 modulation of IL-8 expression in human bronchial epithelial cells exposed to ozone
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کلمات کلیدی
EGFKBMm.o.i.PBSglutathione S-transferase mu 1IL-8GSTM1KGMeGFPRT-PCRHRPROS - ROSInterleukin 8 - اینترلوکین 8BALF - بافتELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاepidermal growth factor - عامل رشد اپیدرمیlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH Bronchoalveolar lavage fluid - مایع لارو برونکلوفلورkeratinocyte basal medium - محتویات پایه کراتینوسیتPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریkeratinocyte growth medium - محیط رشد کراتینوسیتHorseradish peroxidase - پراکسیداز هوررادیشgreen fluorescent protein - پروتئین فلورسنت سبزmultiplicity of infection - چندین عفونتGlutathione ethyl ester - گلوتاتیون اتیل استرReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
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چکیده انگلیسی
Exposure to the major air pollutant ozone can aggravate asthma and other lung diseases. Our recent study in human volunteers has shown that the glutathione S-transferase Mu 1 (GSTM1)-null genotype is associated with increased airway neutrophilic inflammation induced by inhaled ozone. The aim of this study was to examine the effect of GSTM1 modulation on interleukin 8 (IL-8) production in ozone-exposed human bronchial epithelial cells (BEAS-2B) and the underlying mechanisms. Exposure of BEAS-2B cells to 0.4 ppm ozone for 4 h significantly increased IL-8 release, with a modest reduction in intracellular reduced glutathione (GSH). Ozone exposure induced reactive oxygen species (ROS) production and NF-κB activation. Pharmacological inhibition of NF-κB activation or mutation of the IL-8 promoter at the κB-binding site significantly blocked ozone-induced IL-8 production or IL-8 transcriptional activity, respectively. Knockdown of GSTM1 in BEAS-2B cells enhanced ozone-induced NF-κB activation and IL-8 production. Consistently, an ozone-induced overt increase in IL-8 production was detected in GSTM1-null primary human bronchial epithelial cells. In addition, supplementation with reduced GSH inhibited ozone-induced ROS production, NF-κB activation, and IL-8 production. Taken together, GSTM1 deficiency enhances ozone-induced IL-8 production, which is mediated by generated ROS and subsequent NF-κB activation in human bronchial epithelial cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 51, Issue 2, 15 July 2011, Pages 522-529
Journal: Free Radical Biology and Medicine - Volume 51, Issue 2, 15 July 2011, Pages 522-529
نویسندگان
Weidong Wu, Vinod Doreswamy, David Diaz-Sanchez, James M. Samet, Matt Kesic, Lisa Dailey, Wenli Zhang, Ilona Jaspers, David B. Peden,