کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738389 | 1046704 | 2011 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
5-Oxo-ETE is a major oxidative stress-induced arachidonate metabolite in B lymphocytes
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کلمات کلیدی
PBS+5-HETE5-Hydroxyeicosanoid dehydrogenase12-HHTRP-HPLCBCNU5-LO13-HODE - 13-HEAD5-Oxo-ETE - 5-اکسو-ETE5-Lipoxygenase - 5-لیپوکسیژنازHydrogen peroxide - آب اکسیژنه5-oxo-6,8,11,14-eicosatetraenoic acid - اسید 5-اکسو-6،8،11،14-ایکوساتترنوئیکArachidonic acid - اسید آراشیدونیکeicosanoids - ایکوزانوئیدها Oxidative stress - تنش اکسیداتیوFree radicals - رادیکال آزادB lymphocytes - لنفوسیت BLeukotriene B4 - لوکوترین B4Leukotriene - لکوترینpyridine nucleotides - نوکلئوتید پیریدینreversed-phase high-performance liquid chromatography - کروماتوگرافی مایع با کارایی با رزولوشن بالاGlutathione - گلوتاتیون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
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چکیده انگلیسی
B lymphocytes convert arachidonic acid (AA) to the 5-lipoxygenase products leukotriene B4 (LTB4) and 5-hydroxy-6,8,11,14-eicosatetraenoic acid (5-HETE) when subjected to oxidative stress. 5-HETE has little biological activity, but can be oxidized by a selective dehydrogenase in some cells to 5-oxo-6,8,11,14-eicosatetraenoic acid (5-oxo-ETE), a potent eosinophil chemoattractant. We found that CESS cells, a B lymphocyte cell line, convert AA to 5-oxo-ETE and this is selectively stimulated by oxidative stress. In the presence of H2O2, 5-oxo-ETE is a major AA metabolite in these cells (5-oxo-ETE â 5-HETE > LTB4). The cyclooxygenase product 12-hydroxy-5,8,10-heptadecatrienoic acid is also formed, but is not affected by H2O2. Diamide had effects similar to those of H2O2 and both substances had similar effects on human tonsillar B cells. H2O2 also stimulated 5-oxo-ETE formation from its direct precursor 5-HETE in tonsillar B and CESS cells, and this was inhibited by the glutathione reductase inhibitor carmustine. H2O2 concomitantly induced rapid increases in GSSG and NADP+ and reductions in GSH and NADPH. We conclude that oxidative stress stimulates 5-oxo-ETE synthesis in B lymphocytes by two mechanisms: activation of 5-lipoxygenase and increased oxidation of 5-HETE by NADP+-dependent 5-hydroxyeicosanoid dehydrogenase. B lymphocyte-derived 5-oxo-ETE could contribute to eosinophilic inflammation in asthma and other allergic diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 50, Issue 10, 15 May 2011, Pages 1297-1304
Journal: Free Radical Biology and Medicine - Volume 50, Issue 10, 15 May 2011, Pages 1297-1304
نویسندگان
Gail E. Grant, Sylvie Gravel, Julie Guay, Pranav Patel, Bruce D. Mazer, Joshua Rokach, William S. Powell,