کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10738820 1046835 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
On the specificity of 4-amino-5-methylamino-2′,7′-difluorofluorescein as a probe for nitric oxide
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
On the specificity of 4-amino-5-methylamino-2′,7′-difluorofluorescein as a probe for nitric oxide
چکیده انگلیسی
The specificity of 4-amino-5-methylamino-2′,7′-difluorofluorescein (DAF-FM) for nitric oxide was evaluated in in vitro systems. The probe was found fairly specific for nitric oxide. Potential sources of artifacts include the autoxidation of DAF-FM, potentiated by light, and its oxidation by sources of superoxide and peroxyl radicals, leading to fluorescence spectra indistinguishable from those of the nitric oxide adduct. Although DAF-FM reacts with peroxynitrite, this reaction seems to be of secondary importance under quasi-physiological conditions. On the other hand, a simultaneous presence of a nitric oxide source and a superoxide or hydrogen peroxide decreases or increases the fluorescence of DAF-FM, respectively, resulting in biased estimates of nitric oxide production.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 39, Issue 3, 1 August 2005, Pages 327-335
نویسندگان
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