کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738836 | 1046836 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nitrosative stress in an animal model of necrotizing enterocolitis
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کلمات کلیدی
iNOSPBSTNFαLPSIL-6nNOSRNOSSDSNOSL-NNANECPMSFeNOSFfh - FFHl-NAME - L-NAMENω-nitro-l-arginine - N-NITRO-L-ARGININENω-nitro-l-arginine methyl ester - N-Nitro-L-Arginine Methyl Esterneuronal NOS - NOS عصبیinducible NOS - NOS قابل القاییROS - ROSinflammation - التهاب( توروم) Necrotizing enterocolitis - انتروکولیت نکروزانendothelial NOS - اندوتلیال NOSinterleukin-6 - اینترلوکین ۶Breast-fed - تغذیه با شیر مادرtumor necrosis factor-alpha - تومور نکروز عامل آلفاFree radical - رادیکالهای آزاد sodium dodecyl sulfate - سدیم دودسیل سولفاتPlatelet activating factor - عامل فعال کننده پلاکتphenylmethylsulfonyl fluoride - فنیل متیل سولفونیل فلورایدlipopolysaccharide - لیپوپلی ساکاریدPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریNitrosation - نیتروژنNitrite - نیتریتNitric oxide - نیتریک اکسیدnitric oxide synthase - نیتریک اکسید سنتازPAF - نیروی هوایی پاکستانHypoxia - هیپوکسیReactive nitrogen oxide species - گونه اکسید نیتروژن واکنش پذیرReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Necrotizing enterocolitis (NEC) is a disease of newborns characterized by gut barrier failure. We reasoned that upregulation of inducible nitric oxide synthase (iNOS) may result in nitrosative stress and accumulation of nitroso species in the intestine. Newborn rats were either breast-fed (BF), or formula-fed and additionally subjected to hypoxia (FFH). At Day 4 after birth, the distal ilea were harvested and processed for Western blot analysis and measurement of NO-related metabolites. While BF neonates showed normal morphology, FFH neonates developed signs of NEC by Day 4. These pathological changes correlated with upregulation of iNOS and increases in tissue nitrite, nitrosothiol, and nitrosamine concentrations. Enhanced nitroso levels were most prominent in the mucosal layers of the ileum and iNOS inhibition resulted in a significant decrease in both nitroso species and incidence of NEC. In contrast, increased nitrite levels were distributed evenly throughout the ileum and remained unchanged following iNOS inhibition. Similarly, specimens from NEC patients had higher intestinal levels of NO-related metabolites compared to non-NEC controls. This is the first report of tissue levels of nitroso species in the gut of an animal model of NEC and of human specimens. The results suggest that local nitrosative stress contributes to the pathology associated with NEC. Unexpectedly, the NO breakdown product nitrite, previously considered biologically inert, was found to be present throughout the ileal wall, suggesting that cellular NO metabolism is altered significantly in NEC. Whether nitrite plays a protective or deleterious role remains to be investigated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 39, Issue 11, 1 December 2005, Pages 1428-1437
Journal: Free Radical Biology and Medicine - Volume 39, Issue 11, 1 December 2005, Pages 1428-1437
نویسندگان
Ruben Zamora, Nathan S. Bryan, Patricia Boyle, Catarina Wong, Alexandra B. Milsom, Ronald Jaffe, Martin Feelisch, Henri R. Ford,