کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10739325 | 1046871 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Vitamin C homeostasis in skeletal muscle cells
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کلمات کلیدی
SVCT1PBSD-MEMFBSdeoxyglucoseSVCT2AFRGLUTBSODTTDTNBGSHRT-PCR5,5′-dithiobis(2-nitrobenzoic acid) - 5،5'-dithiobis (2-nitrobenzoic acid)Dulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoROS - ROSAscorbic acid - آسکوربیک اسیدDehydroascorbic acid - اسید Dehydroascorbicbuthionine sulfoximine - بوته یون سولفسیمیمDHA - دوکوساهگزائنوئیک اسیدdithiothreitol - دیتیوتریتولFree radicals - رادیکال آزادAscorbyl free radical - رادیکال آزاد آسکوربیلfetal bovine serum - سرم جنین گاوDog - سگSkeletal muscle - عضله اسکلتیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریreverse transcriptase polymerase chain reaction - واکنش زنجیره ای پلی مراز ترانس کریتاز معکوسreduced glutathione - کاهش گلوتاتیونGlutathione - گلوتاتیونReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In skeletal muscle, vitamin C not only enhances carnitine biosynthesis but also protects cells against ROS generation induced by physical exercise. The ability to take up both ascorbic and dehydroascorbic acid from the extracellular environment, together with the ability to recycle the intracellular vitamin, maintains high cellular stores of ascorbate. In this study, we examined vitamin C transport and recycling, by using the mouse C2C12 and rat L6C5 muscle cell lines, which exhibit different sensitivity to oxidative stress and GSH metabolism. We found that: (1) both cell lines express SVCT2, whereas SVCT1 is expressed at very low levels only in proliferating L6C5 cells; furthermore L6C5 myoblasts are more efficient in ascorbic acid transport than C2C12 myoblasts; (2) C2C12 cells are more efficient in dehydroascorbic acid transport and ascorbyl free radical/dehydroascorbic acid reduction; (3) differentiation is paralleled by decreased ascorbic acid and dehydroascorbic acid transport and reduction and increased ascorbyl free radical reduction; (4) differentiated cells are more responsive to oxidative stress induced by glutathione depletion; indeed, myotubes showed increased SVCT2 expression and thioredoxin reductase-mediated dehydroascorbic acid reduction. From our data, SVCT2 and NADPH-thioredoxin-dependent DHA reduction appears to belong to an inducible system activated in response to oxidative stress.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 38, Issue 7, 1 April 2005, Pages 898-907
Journal: Free Radical Biology and Medicine - Volume 38, Issue 7, 1 April 2005, Pages 898-907
نویسندگان
Isabella Savini, Maria Valeria Catani, Guglielmo Duranti, Roberta Ceci, Stefania Sabatini, Luciana Avigliano,