کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10739327 | 1046871 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hydroxytyrosol, a natural antioxidant from olive oil, prevents protein damage induced by long-wave ultraviolet radiation in melanoma cells
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کلمات کلیدی
UVBCOMTFCSS-adenosylhomocysteineDCFH-DAAdoMet2′7′-dichlorofluoresceinAdoHcyTBARSUVACatechol-O-methyltransferaseProtein L-isoaspartyl methyltransferaseDcfHomovanillic alcoholDOPET3,4-dihydroxyphenylethanolPBSROS - ROSPIMT - TMIPProtein damage - آسیب پروتئینS-adenosylmethionine - اس-ادنوزیل متیونینUVA radiation - اشعه ماوراء بنفشstandard error - خطای استانداردFree radicals - رادیکال آزادfetal calf serum - سرم گوساله جنینMelanoma cells - سلول های ملانومProtein deamidation - غلظت پروتئینPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریHydroxytyrosol - هیدروکسی تیروسولHVA - چهhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراHPLC - کروماتوگرافی مایعی کاراReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Previous studies showed that long-wave ultraviolet (UVA) radiation induces severe skin damage through the generation of reactive oxygen species and the depletion of endogenous antioxidant systems. Recent results from our laboratory indicate a dramatic increase of both lipid peroxidation products (TBARS) and abnormal l-isoaspartyl residues, marker of protein damage, in UVA-irradiated human melanoma cells. In this study, the effects of hydroxytyrosol (DOPET), the major antioxidant compound present in olive oil, on UVA-induced cell damages, have been investigated, using a human melanoma cell line (M14) as a model system. In UVA-irradiated M14 cells, a protective effect of DOPET in preventing the uprise of typical markers of oxidative stress, such as TBARS and 2â²7â²-dichlorofluorescein (DCF) fluorescence intensity, was observed. In addition, DOPET prevents the increase of altered l-isoAsp residues induced by UVA irradiation. These protective effects are dose dependent, reaching the maximum at 400 μM DOPET. At higher concentrations, DOPET causes an arrest of M14 cell proliferation and acts as a proapoptotic stimulus by activating caspase-3 activity. In the investigated model system, DOPET is quantitatively converted into its methylated derivative, endowed with a radical scavenging ability comparable to that of its parent compound. These findings are in line with the hypothesis that the oxidative stress plays a major role in mediating the UVA-induced protein damage. Results suggest that DOPET may exerts differential effects on melanoma cells according to the dose employed and this must always be taken into account when olive oil-derived large consumer products, including cosmetics and functional foods, are employed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 38, Issue 7, 1 April 2005, Pages 908-919
Journal: Free Radical Biology and Medicine - Volume 38, Issue 7, 1 April 2005, Pages 908-919
نویسندگان
Stefania D'Angelo, Diego Ingrosso, Valentina Migliardi, Alvara Sorrentino, Giovanna Donnarumma, Adone Baroni, Lucia Masella, Maria Antonietta Tufano, Marcello Zappia, Patrizia Galletti,