کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10739656 | 1046883 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Vitamin C inhibits platelet expression of CD40 ligand
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PBSSoluble CD40LmAbFITCsCD40LCD40LPrPBSA - BSAbovine serum albumin - آلبومین سرم گاوfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتCD40 ligand - لیگاند CD40Phosphate-buffered saline - محلول نمک فسفات با خاصیت بافریArbitrary Unit - واحد خودآموزvitamin C - ویتامین سیMonoclonal antibodies - پادتنهای تَکتیرهplatelet-rich plasma - پلاسمای غنی از پلاکت، PRP، پی آر پیPlatelets - پلاکت هاReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Vitamin C inhibits platelet expression of CD40 ligand Vitamin C inhibits platelet expression of CD40 ligand](/preview/png/10739656.png)
چکیده انگلیسی
Upon stimulation with agonists, platelets express CD40 ligand (CD40L), a transmembrane protein implicated in the initiation and progression of atherosclerotic disease. We have recently discovered that oxidative stress plays a major role in platelet CD40L expression. In this study, we sought to determine whether vitamin C, a known antioxidant, is able to influence platelet CD40L expression. In vitro experiments were done by stimulating platelets with collagen in the presence or absence of vitamin C (50-100 μM) or vehicle as control. An in vivo study was done in 10 healthy subjects who were randomized to intravenous infusion of placebo or 1 g vitamin C for 45 min in a crossover design. At the end of infusion platelet CD40L and O2- were measured. The in vitro study demonstrated that vitamin C dose dependently inhibited platelet CD40L expression without affecting agonist-induced platelet aggregation. In subjects treated with placebo no changes of platelet CD40L and O2- were observed; conversely, vitamin C infusion caused a significant and parallel decrease of platelet O2- (â70%, P < 0.001) and CD40L (â68%, P < 0.001). Platelet aggregation was not modified by either treatment. This study suggests that water-soluble antioxidants, which scavenge superoxide radicals, may reduce platelet CD40L expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 38, Issue 12, 15 June 2005, Pages 1662-1666
Journal: Free Radical Biology and Medicine - Volume 38, Issue 12, 15 June 2005, Pages 1662-1666
نویسندگان
Pasquale Pignatelli, Valerio Sanguigni, Sciacca Giordano Paola, Emanuela Lo Coco, Luisa Lenti, Francesco Violi,