کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10748947 1050284 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of an interaction between EI and a histidine kinase-response regulator hybrid protein in Gluconobacter oxydans
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Identification of an interaction between EI and a histidine kinase-response regulator hybrid protein in Gluconobacter oxydans
چکیده انگلیسی
Gluconobacter oxydans may contain an incomplete phosphoenolpyruvate: carbohydrate phosphotransferase system consisting of three components - EI, HPr and EIIA, while the function of individual members of the system remains unknown. In this research, a specific interaction between EI and a histidine kinase-response regulator hybrid protein was screened by yeast two-hybrid assay, and the interaction was further identified with GST pull-down assay and bimolecular fluorescence complementation assay in vitro and in vivo, respectively. As the histidine kinase-response regulator hybrid protein serves as a member of two-component system in G. oxydans, its interaction with EI implied that PTS may play certain roles in bacteria under stress.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 470, Issue 2, 5 February 2016, Pages 331-335
نویسندگان
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