کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10749032 | 1050285 | 2016 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolated human islets require hyperoxia to maintain islet mass, metabolism, and function
ترجمه فارسی عنوان
جزایر انسانی نیاز به هیپراکسی برای حفظ توده جزیی، متابولیسم و عملکرد دارد
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
هیپوکسیا، هیپوکسیا، زنده ماندن، ترشح انسولین، حجم جزیره، میزان مصرف اکسیژن،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Pancreatic islet transplantation has been recognized as an effective treatment for Type 1 diabetes; however, there is still plenty of room to improve transplantation efficiency. Because islets are metabolically active they require high oxygen to survive; thus hypoxia after transplant is one of the major causes of graft failure. Knowing the optimal oxygen tension for isolated islets would allow a transplant team to provide the best oxygen environment during pre- and post-transplant periods. To address this issue and begin to establish empirically determined guidelines for islet maintenance, we exposed in vitro cultured islets to different partial oxygen pressures (pO2) and assessed changes in islet volume, viability, metabolism, and function. Human islets were cultured for 7 days in different pO2 media corresponding to hypoxia (90 mmHg), normoxia (160 mmHg), and hyerpoxia (270 or 350 mmHg). Compared to normoxia and hypoxia, hyperoxia alleviated the loss of islet volume, maintaining higher islet viability and metabolism as measured by oxygen consumption and glucose-stimulated insulin secretion responses. We predict that maintaining pre- and post-transplanted islets in a hyperoxic environment will alleviate islet volume loss and maintain islet quality thereby improving transplant outcomes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 470, Issue 3, 12 February 2016, Pages 534-538
Journal: Biochemical and Biophysical Research Communications - Volume 470, Issue 3, 12 February 2016, Pages 534-538
نویسندگان
Hirotake Komatsu, Dongyang Kang, Leonard Medrano, Alyssa Barriga, Daniel Mendez, Jeffrey Rawson, Keiko Omori, Kevin Ferreri, Yu-Chong Tai, Fouad Kandeel, Yoko Mullen,