کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10749170 | 1050286 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Mutation of the gene encoding the ribonuclease P RNA in the hyperthermophilic archaeon Thermococcus kodakarensis causes decreased growth rate and impaired processing of tRNA precursors
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کلمات کلیدی
qRT-PCRCBPASWRNase P5-FOACalmodulin-binding peptideThermococcus kodakarensisPhopRNAPre-tRNA processingquantitative RT-PCR - RT-PCR کمیpre-tRNA - tRNA هاartificial sea water - آب دریا مصنوعیHyperthermophilic archaea - آرکه Hyperthermophilic5-fluoroorotic acid - اسید 5-فلوروروتیکribonuclease P - ریبونوکلئاز P
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Mutation of the gene encoding the ribonuclease P RNA in the hyperthermophilic archaeon Thermococcus kodakarensis causes decreased growth rate and impaired processing of tRNA precursors Mutation of the gene encoding the ribonuclease P RNA in the hyperthermophilic archaeon Thermococcus kodakarensis causes decreased growth rate and impaired processing of tRNA precursors](/preview/png/10749170.png)
چکیده انگلیسی
Ribonuclease P (RNase P) catalyzes the processing of 5â² leader sequences of tRNA precursors in all three phylogenetic domains. RNase P also plays an essential role in non-tRNA biogenesis in bacterial and eukaryotic cells. For archaeal RNase Ps, additional functions, however, remain poorly understood. To gain insight into the biological function of archaeal RNase Ps in vivo, we prepared archaeal mutants KUWÎP3, KUWÎP8, and KUWÎP16, in which the gene segments encoding stem-loops containing helices, respectively, P3, P8 and P16 in RNase P RNA (TkopRNA) of the hyperthermophilic archaeon Thermococcus kodakarensis were deleted. Phenotypic analysis showed that KUWÎP3 and KUWÎP16 grew slowly compared with wild-type T. kodakarensis KUW1, while KUWÎP8 displayed no difference from T. kodakarensis KUW1. RNase P isolated using an affinity-tag from KUWÎP3 had reduced pre-tRNA cleavage activity compared with that from T. kodakarensis KUW1. Moreover, quantitative RT-PCR (qRT-PCR) and Northern blots analyses of KUWÎP3 showed greater accumulation of unprocessed transcripts for pre-tRNAs than that of T. kodakarensis KUW1. The current study represents the first attempt to prepare mutant T. kodakarensis with impaired RNase P for functional investigation. Comparative whole-transcriptome analysis of T. kodakarensis KUW1 and KUWÎP3 should allow for the comprehensive identification of RNA substrates for archaeal RNase Ps.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 468, Issue 4, 25 December 2015, Pages 660-665
Journal: Biochemical and Biophysical Research Communications - Volume 468, Issue 4, 25 December 2015, Pages 660-665
نویسندگان
Toshifumi Ueda, Sonoko Ishino, Kotaro Suematsu, Takashi Nakashima, Yoshimitsu Kakuta, Yutaka Kawarabayasi, Yoshizumi Ishino, Makoto Kimura,