کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10749349 | 1050289 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Crystal structure of nanoKAZ: The mutated 19Â kDa component of Oplophorus luciferase catalyzing the bioluminescent reaction with coelenterazine
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The 19Â kDa protein (KAZ) of Oplophorus luciferase is a catalytic component, that oxidizes coelenterazine (a luciferin) with molecular oxygen to emit light. The crystal structure of the mutated 19Â kDa protein (nanoKAZ) was determined at 1.71Â Ã
resolution. The structure consists of 11 antiparallel β-strands forming a β-barrel that is capped by 4 short α-helices. The structure of nanoKAZ is similar to those of fatty acid-binding proteins (FABPs), even though the amino acid sequence similarity was very low between them. The coelenterazine-binding site and the catalytic site for the luminescence reaction might be in a central cavity of the β-barrel structure.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 470, Issue 1, 29 January 2016, Pages 88-93
Journal: Biochemical and Biophysical Research Communications - Volume 470, Issue 1, 29 January 2016, Pages 88-93
نویسندگان
Yuri Tomabechi, Takamitsu Hosoya, Haruhiko Ehara, Shun-ichi Sekine, Mikako Shirouzu, Satoshi Inouye,