کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10750820 | 1050304 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
ATP selection in a random peptide library consisting of prebiotic amino acids
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Based upon many theoretical findings on protein evolution, we proposed a ligand-selection model for the origin of proteins, in which the most ancient proteins originated from ATP selection in a pool of random peptides. To test this ligand-selection model, we constructed a random peptide library consisting of 15 types of prebiotic amino acids and then used cDNA display to perform six rounds of in vitro selection with ATP. By means of next-generation sequencing, the most prevalent sequence was defined. Biochemical and biophysical characterization of the selected peptide showed that it was stable and foldable and had ATP-hydrolysis activity as well.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 466, Issue 3, 23 October 2015, Pages 400-405
Journal: Biochemical and Biophysical Research Communications - Volume 466, Issue 3, 23 October 2015, Pages 400-405
نویسندگان
Shou-Kai Kang, Bai-Xue Chen, Tian Tian, Xi-Shuai Jia, Xin-Yi Chu, Rong Liu, Peng-Fei Dong, Qing-Yong Yang, Hong-Yu Zhang,