کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10751550 | 1050314 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
GATA-1 directly regulates Nanog in mouse embryonic stem cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: GATA-1 directly regulates Nanog in mouse embryonic stem cells GATA-1 directly regulates Nanog in mouse embryonic stem cells](/preview/png/10751550.png)
چکیده انگلیسی
Nanog safeguards pluripotency in mouse embryonic stem cells (mESCs). Insight into the regulation of Nanog is important for a better understanding of the molecular mechanisms that control pluripotency of mESCs. In a silico analysis, we identify four GATA-1 putative binding sites in Nanog proximal promoter. The Nanog promoter activity can be significantly repressed by ectopic expression of GATA-1 evidenced by a promoter reporter assay. Mutation studies reveal that one of the four putative binding sites counts for GATA-1 repressing Nanog promoter activity. Direct binding of GATA-1 on Nanog proximal promoter is confirmed by electrophoretic mobility shift assay and chromatin immunoprecipitation. Our data provide new insights into the expanded regulatory circuitry that coordinates Nanog expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 465, Issue 3, 25 September 2015, Pages 575-579
Journal: Biochemical and Biophysical Research Communications - Volume 465, Issue 3, 25 September 2015, Pages 575-579
نویسندگان
Wen-Zhong Li, Zhi-Ying Ai, Zhi-Wei Wang, Lin-Lin Chen, Ze-Kun Guo, Yong Zhang,