کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10751839 | 1050319 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
ABT-737 sensitizes curcumin-induced anti-melanoma cell activity through facilitating mPTP death pathway
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کلمات کلیدی
adenine nucleotide translocator 1ABT-737Sanglifehrin ACyclophilin-D(MPTP)Mitochondrial permeability transition pore (mPTP) - افت معدنی نفوذپذیری میتوکندری (mPTP)mitochondrial permeability transition pore - انتقال نفوذی میتوکندری منفی استMelanoma - خال سرطانی یا ملانوماcyclosporin A - سیکلوسپورین AMitochondrial membrane potential - پتانسیل غشای میتوکندریvoltage-dependent anion channel - کانال آنیون وابسته به ولتاژCurcumin - کورکومین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
In the current study, we studied the potential role of ABT-737, a novel Bcl-2 inhibitor, on curcumin-induced anti-melanoma cell activity in vitro. The associated mechanisms were also investigated. We demonstrated that ABT-737 significantly sensitized curcumin-induced activity against melanoma cells (WM-115 and B16 lines), resulting in substantial cell death and apoptosis with co-administration. At the molecular level, curcumin and ABT-737 synergistically induced mitochondrial permeability transition pore (mPTP) opening in melanoma cells, the latter was evidenced by mitochondrial membrane potential (MPP) reduction and mitochondrial complexation between cyclophilin-D (CyPD) and adenine nucleotide translocator 1 (ANT-1). Significantly, mPTP blockers, including cyclosporin A and sanglifehrin A, remarkably inhibited curcumin and ABT-737 co-administration-induced cytotoxicity against melanoma cells. Meanwhile, siRNA-mediated knockdown of CyPD or ANT-1, the two key components of mPTP, alleviated WM-116 cell death by the co-treatment. Collectively, we show that ABT-737 sensitizes curcumin-induced anti-melanoma cell activity probably through facilitating mPTP death pathway. ABT-737 could be further investigated as a potential curcumin adjuvant in melanoma and other cancer treatment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 464, Issue 1, 14 August 2015, Pages 286-291
Journal: Biochemical and Biophysical Research Communications - Volume 464, Issue 1, 14 August 2015, Pages 286-291
نویسندگان
Teng Yu, Chao Chen, Yang Sun, Hui Sun, Tian-hang Li, Jin Meng, Xianhua Shi,