کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10752364 | 1050326 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Spontaneous l-glutamate release enhancement in rat substantia gelatinosa neurons by (â)-carvone and (+)-carvone which activate different types of TRP channel
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Transient receptor potential (TRP) channels in the spinal dorsal horn lamina II (substantia gelatinosa; SG), which are involved in the modulation of nociceptive transmission, have not yet been fully examined in property. Activation of the TRP channels by various plant-derived chemicals results in an increase in the spontaneous release of l-glutamate onto the SG neurons. We examined the effects of a monoterpene ketone (â)-carvone (contained in spearmint) and its stereoisomer (+)-carvone (in caraway) on glutamatergic spontaneous excitatory transmission in SG neurons of adult rat spinal cord slices by using the whole-cell patch-clamp technique. (â)-Carvone and (+)-carvone increased the frequency of spontaneous excitatory postsynaptic current (sEPSC) in a reversible and concentration-dependent manner with a small increase in its amplitude. Half-maximal effective concentrations of (â)-carvone and (+)-carvone in increasing sEPSC frequency were 0.70Â mM and 0.72Â mM, respectively. The (â)-carvone but not (+)-carvone activity was inhibited by a TRPV1 antagonist capsazepine. On the other hand, the (+)-carvone but not (â)-carvone activity was inhibited by a TRPA1 antagonist HC-030031. These results indicate that (â)-carvone and (+)-carvone activate TRPV1 and TRPA1 channels, respectively, resulting in an increase in spontaneous l-glutamate release onto SG neurons, with almost the same efficacy. Such a difference in TRP activation between the stereoisomers may serve to know the properties of TRP channels in the SG.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 459, Issue 3, 10 April 2015, Pages 498-503
Journal: Biochemical and Biophysical Research Communications - Volume 459, Issue 3, 10 April 2015, Pages 498-503
نویسندگان
Qin Kang, Chang-Yu Jiang, Tsugumi Fujita, Eiichi Kumamoto,