کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10753362 1050339 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Double-stranded RNA transcribed from vector-based oligodeoxynucleotide acts as transcription factor decoy
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Double-stranded RNA transcribed from vector-based oligodeoxynucleotide acts as transcription factor decoy
چکیده انگلیسی
In this study, we designed a short hairpin RNA vector-based oligodeoxynucleotide (VB-ODN) carrying transcription factor (TF) consensus sequence which could function as a decoy to block TF activity. Specifically, VB-ODN for Nuclear factor-κB (NF-κB) could inhibit cell viability and decrease downstream gene expression in HEK293 cells without affecting expression of NF-κB itself. The specific binding between VB-ODN produced double-stranded RNA and NF-κB was evidenced by electrophoretic mobility shift assay. Moreover, similar VB-ODNs designed for three other TFs also inhibit their downstream gene expression but not that of themselves. Our study provides a new design of decoy for blocking TF activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 457, Issue 2, 6 February 2015, Pages 221-226
نویسندگان
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