کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10753482 | 1050341 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of hypothalamic murine and human melanocortin 3 receptor transcript structure
ترجمه فارسی عنوان
ارزیابی ساختار رونویسی گیرنده هیپوتالاموس موش و ملانوکورتین 3
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
The melanocortin 3 receptor (MC3R) is involved in regulation of energy homeostasis. However, its transcript structure is not well understood. We therefore studied initiation and termination sites for hypothalamic murine Mc3r and human MC3R transcripts. Rapid Amplification of cDNA Ends (RACE) was performed for the 5â² and 3â² ends of murine and human hypothalamic RNA. 5â² RACE experiments using hypothalamic murine RNA indicated mouse hypothalamus expresses two major Mc3r transcription start sites: one with a 5â² UTR approximately 368 bases in length and another previously unknown transcript with a 5â² UTR approximately 440 bases in length. 5â² RACE experiments using human hypothalamic RNA identified a 5â² UTR beginning 533 bases upstream of the start codon with a 248 base splice. 3â² RACE experiments using hypothalamic murine RNA indicated the 3â² UTR terminates approximately 1286 bases after the translational stop codon, with a previously unknown 787 base splice between consensus splice donor and acceptor sites. 3â² RACE experiments using human MC3R transcript indicated the 3â² UTR terminates approximately 115-160 bases after the translational stop codon. These data provide insight into melanocortin 3 receptor transcript structure.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 454, Issue 1, 7 November 2014, Pages 234-238
Journal: Biochemical and Biophysical Research Communications - Volume 454, Issue 1, 7 November 2014, Pages 234-238
نویسندگان
Dezmond C. Taylor-Douglas, Arunabha Basu, Ryan M. Gardner, Sender Aspelund, Xin Wen, Jack A. Yanovski,