کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10753768 | 1050345 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Surface features of the lipid droplet mediate perilipin 2 localization
ترجمه فارسی عنوان
ویژگی های سطح قطرات چربی متوسط پرولیپین 2
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کلمات کلیدی
LaurdanPoPCDOPCMerocyanine 5406-dodecanoyl-2-dimethylaminonaphthalene1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine - 1-پالمیتویل-2-اولئویل-اسن-گلیسرو-3-فسفوشولین1,2-dioleoyl-sn-glycero-3-phosphocholine - 1،2-dioleoyl-sn-glycero-3-phosphocholineMC540 - MC 540large unilamellar vesicle - بزرگ کیسه بیضهPhospholipid monolayer - تک فاز فسفولیپیدlipid droplet - قطره چربیLUV - لووperilipin 2 - پریلیپین 2
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
All eukaryotic organisms store excess lipid in intracellular lipid droplets. These dynamic structures are associated with and regulated by numerous proteins. Perilipin 2, an abundant protein on most lipid droplets, promotes neutral lipid accumulation in lipid droplets. However, the mechanism by which perilipin 2 binds to and remains anchored on the lipid droplet surface is unknown. Here we identify features of the lipid droplet surface that influence perilipin 2 localization. We show that perilipin 2 binding to the lipid droplet surface requires both hydrophobic and electrostatic interactions. Reagents that disrupt these interactions also decrease binding. Moreover, perilipin 2 binding does not depend on other lipid droplet-associated proteins but is influenced by the lipid composition of the surface. Perilipin 2 binds to synthetic vesicles composed of dioleoylphosphatidylcholine, a phospholipid with unsaturated acyl chains. Decreasing the temperature of the binding reaction, or introducing phospholipids with saturated acyl chains, decreases binding. We therefore demonstrate a role for surface lipids and acyl chain packing in perilipin 2 binding to lipid droplets. The ability of the lipid droplet phospholipid composition to impact protein binding may link changes in nutrient availability to lipid droplet homeostasis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 452, Issue 3, 26 September 2014, Pages 422-427
Journal: Biochemical and Biophysical Research Communications - Volume 452, Issue 3, 26 September 2014, Pages 422-427
نویسندگان
Arthur Sletten, Alison Seline, Andrew Rudd, Michelle Logsdon, Laura L. Listenberger,