کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10754261 | 1050353 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High-resolution crystal structure reveals a HEPN domain at the C-terminal region of S. cerevisiae RNA endonuclease Swt1
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کلمات کلیدی
N-terminalNPCsingle wavelength anomalous dispersionmRNPSAXSC-terminal - ترمینال CTREX - تی رکسSeMet - خودشانmessenger ribonucleoprotein particle - ذرات ریبونولوپروتئین رسولCrystal structure - ساختار کریستالیSAD - غمگینnuclear pore complex - مجتمع مخرب هسته ایsmall angle X-ray Scattering - پراکندگی اشعه ایکس زاویه کوچک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: High-resolution crystal structure reveals a HEPN domain at the C-terminal region of S. cerevisiae RNA endonuclease Swt1 High-resolution crystal structure reveals a HEPN domain at the C-terminal region of S. cerevisiae RNA endonuclease Swt1](/preview/png/10754261.png)
چکیده انگلیسی
Swt1 is an RNA endonuclease that plays an important role in quality control of nuclear messenger ribonucleoprotein particles (mRNPs) in eukaryotes; however, its structural details remain to be elucidated. Here, we report the crystal structure of the C-terminal (CT) domain of Swt1 from Saccharomyces cerevisiae, which shares common characteristics of higher eukaryotes and prokaryotes nucleotide binding (HEPN) domain superfamily. To study in detail the full-length protein structure, we analyzed the low-resolution architecture of Swt1 in solution using small angle X-ray scattering (SAXS) method. Both the CT domain and middle domain exhibited a good fit upon superimposing onto the molecular envelope of Swt1. Our study provides the necessary structural information for detailed analysis of the functional role of Swt1, and its importance in the process of nuclear mRNP surveillance.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 453, Issue 4, 31 October 2014, Pages 826-832
Journal: Biochemical and Biophysical Research Communications - Volume 453, Issue 4, 31 October 2014, Pages 826-832
نویسندگان
Shuxia Peng, Ke Zhou, Wenjia Wang, Zengqiang Gao, Yuhui Dong, Quansheng Liu,