کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10754651 | 1050359 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
c-Myb negatively regulates Ras signaling through induction of dual phosphatase MKP-3 in NIH3T3 cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: c-Myb negatively regulates Ras signaling through induction of dual phosphatase MKP-3 in NIH3T3 cells c-Myb negatively regulates Ras signaling through induction of dual phosphatase MKP-3 in NIH3T3 cells](/preview/png/10754651.png)
چکیده انگلیسی
Mitogen-activated protein kinase phosphatase-3 (MKP-3) negatively regulates ERK1/2 MAPK in a feedback loop. However, little is known about the molecular mechanism by which Ras signaling induces MKP-3 expression. In the present study, we demonstrate that exogenous expression of constitutively active H-Ras increases the level of MKP-3 mRNA. A transfection study using a series of MKP-3 promoter deletion constructs revealed that the c-Myb binding site is required for Ras-induced transcriptional activation of the MKP-3 gene promoter. Furthermore, we show that c-Myb directly binds to the MKP-3 promoter, as revealed by electrophoretic mobility shift assay and chromatin immunoprecipitation. Knock-down of c-Myb expression using siRNA abrogated Ras-induced MKP-3 promoter activity. These findings propose a novel mechanism through which Ras signaling activates c-Myb-dependent transcriptional activation of the MKP-3 gene.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 450, Issue 2, 25 July 2014, Pages 1032-1037
Journal: Biochemical and Biophysical Research Communications - Volume 450, Issue 2, 25 July 2014, Pages 1032-1037
نویسندگان
Young Jae Park, Jong Min Lee, Mi So Lee, Young Ho Kim, Soon Young Shin,