کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10755012 1050365 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The functional expression and motile properties of recombinant outer arm dynein from Tetrahymena
ترجمه فارسی عنوان
بیان کارکردی و خواص حرکتی بازو خارجی نوترکیب دیینین از تتراهیمن
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Cilia and flagella are motile organelles that play various roles in eukaryotic cells. Ciliary movement is driven by axonemal dyneins (outer arm and inner arm dyneins) that bind to peripheral microtubule doublets. Elucidating the molecular mechanism of ciliary movement requires the genetic engineering of axonemal dyneins; however, no expression system for axonemal dyneins has been previously established. This study is the first to purify recombinant axonemal dynein with motile activity. In the ciliated protozoan Tetrahymena, recombinant outer arm dynein purified from ciliary extract was able to slide microtubules in a gliding assay. Furthermore, the recombinant dynein moved processively along microtubules in a single-molecule motility assay. This expression system will be useful for investigating the unique properties of diverse axonemal dyneins and will enable future molecular studies on ciliary movement.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 447, Issue 4, 16 May 2014, Pages 596-601
نویسندگان
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