کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10755123 | 1050368 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Resistin regulates the expression of plasminogen activator inhibitor-1 in 3T3-L1 adipocytes
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کلمات کلیدی
Smad3carbohydrate responsive element-binding proteinChREBPPAI-1GLUT4PPARγPP2API3KC/EBPα - C / EBPαCCAAT/enhancer-binding protein α - CCAAT / پروتئین اتصال دهنده تقویت کننده αAdipokine - آدپیکینAdipocyte - آدیپوسیتAkt - آکتResistin - رزیستینphosphoinositide 3-kinase - فسفینوزیتید 3-کینازPlasminogen activator inhibitor-1 - مهار کننده فعال کننده پلاسمینوژن-1Glucose transporter type 4 - نوع حمل گلوکز 4protein phosphatase 2A - پروتئین فسفاتاز 2Aperoxisome proliferator activated receptor γ - گیرنده پروتئینزا پراکسیوم فعال γ
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Previously, we found that resistin regulates lipid metabolism via carbohydrate responsive element-binding protein (ChREBP) during adipocyte maturation (Ikeda et al., 2013) [6]. In this study, to clarify the relationship between expression of resistin and PAI-1, PAI-1 expression in differentiated 3T3-L1 adipocytes was measured after transfection with anti-resistin siRNA. We found that PAI-1 gene expression and secreted PAI-1 protein were significantly decreased by resistin knockdown. Furthermore, phosphorylation of Akt, which can inhibit PAI-1 expression, was accelerated and the activity of protein phosphatase 2A (PP2A) was suppressed in resistin knockdown 3T3-L1 adipocytes. In addition, the expression of glucose transporter type 4, a ChREBP target gene, was reduced and was associated with inhibition of PP2A. The addition of culture medium collected from COS7 cells transfected with a resistin expression plasmid rescued the suppression of PAI-1 expression in resistin knockdown 3T3-L1 adipocytes. Our findings suggest that resistin regulates PAI-1 expression in 3T3-L1 adipocytes via Akt phosphorylation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 448, Issue 2, 30 May 2014, Pages 129-133
Journal: Biochemical and Biophysical Research Communications - Volume 448, Issue 2, 30 May 2014, Pages 129-133
نویسندگان
Yoshito Ikeda, Hiroyuki Tsuchiya, Susumu Hama, Kazuaki Kajimoto, Kentaro Kogure,